Welcome to the Bai Lab

The Bai Lab uses a variety of strategies to understand the mechanism of gene regulation by chromatin structure at different levels. Currently, we are working on two main projects: (1) to identify and characterize “pioneer factors” that can lead to nucleosome depletion and (2) to mechanistically dissect long-distance chromosomal interactions that regulate gene expression. We measure gene expression in single live cells to probe how these chromatin features affect gene expression in terms of the average level, cell-to-cell variability (noise), and dynamics. We are using budding yeast as our primary model system, but we are venturing into the mammalian cells as well. Method-wise, we are using a combination of imaging, genetics, genomics, and computational methods. We are also developing new genetics and genomics tools for the projects above.

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Lab News

11/22: Hungyo’s method paper “Predicting Nucleosome Positioning Using Statistical Equilibrium Models in Budding Yeast” is accepted by Star Protocol.

10/22: Congratulations to Hengye and Fan for successfully defend their theses!

10/22: Cheng, Holly, and Lucy attended ASBMB Transcriptional Regulation Chromatin and RNA Polymerase II Conference and presented our recent work.

08/22: Another collaborative work with Shixin Liu’s lab, “Origin recognition complex harbors an intrinsic nucleosome remodeling activity”, is accepted by PNAS.

08/22: Lucy presented a guest lecture in Cold Spring Harbor Yeast Genetics & Genomics course introducing our ISO methods.

07/22: Lucy’s collaborative work with Shixin Liu’s lab, “Nucleosome-directed replication origin licensing independent of a consensus DNA sequence”, is accepted by Nature Communications.

07/22: Hengye’s work, “Partitioned Usage of Chromatin Remodelers by Nucleosome-Displacing Factors”, is accepted by Cell Reports.

06/22: The project Lucy worked on during her sabbatical, “Mechanisms underlying WNT-mediated priming of human embryonic stem cells”, is accepted by Development.

Funding

Bai lab is grateful for fundings from NIH, NSF, PSU, and previous fellowship supports.